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Old 03-28-2011, 09:05 PM   #15192
ACt
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Re: Narutard chat thread

I had a pretty interesting day. I got up around 7:10 am and broke my fast with a bowl of corn flakes topped with strawberries, while groaning at the local radio horribly simplifying the issues surrounding Canada's upcoming election rather than trying to intelligently find a narrative that could make people care enough to vote. They even wasted 10 minutes talking about how the old white party leaders are forced into the Twitter world. Standard hour commute via bus and subway, where I started a new book by Raymond E. Feist, a rather talented fantasy writer who I am drawing some inspiration for my own work. Got to work, and started up a fresh gel electrophoresis to run some murine cardiac tissue samples against some Jurkat T Cell line proteins, so I could compare various systems that I'm attempting to expand to the cardiac field as a part of my thesis. While the gel ran, I worked on a boring scholarship application that I probably won't get, but you never truly know until you apply. Dealt with a few other labmates, who were up in arms because someone contaminated the cell culture incubator, which put a bunch of them behind in their work. Luckily, it wasn't the one my cells were in, so none of them could blame me. Most importantly, my cells are fine. Had a six inch meatball sub for lunch, and spent the afternoon going over some data analysis, as the completed gel was transferred to a membrane. Took some time to wash out some mouse hearts, lungs and kidneys with sterilized PBS, to rinse out the fixation factor, PFA. I should embed them in parafin on Wednesday. As the day wore down, I sent up to check on my mouse colony, to find one mouse is suffering from malocclusion, which they had begun treating without my say. I may need to figure out what they are doing and see if the costs are warranted. Added a primary antibody to my membrane and left for home. Took an hour and half to get home, because the transit always slows in the afternoon, but got home in time to clean the kitchen, make a tasty green curry with shrimp, and eat it with the wife, who worked two hours later than I did, because she's a dedicated teacher, while watching a House rerun. I still don't understand what they are doing with that show this season. And now, I'm typing up my day to see if anything interesting happens or it is just another bedtime story for Miburo.

Probably the later.
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